# Chromogenic Endotoxin Quantification Kit for Accurate Bacterial Toxin Measurement
## Introduction to Endotoxins and Their Impact
Endotoxins, also known as lipopolysaccharides (LPS), are toxic components found in the outer membrane of Gram-negative bacteria. These molecules can trigger strong immune responses in humans and animals, making their detection and quantification crucial in various industries, including pharmaceuticals, medical devices, and biotechnology.
## What is the Chromogenic Endotoxin Quant Kit?
The Chromogenic Endotoxin Quant Kit is a highly sensitive and specific tool designed for the accurate measurement of bacterial endotoxins. This innovative kit utilizes a chromogenic substrate that reacts with endotoxins to produce a color change, which can be measured spectrophotometrically.
### Key Features of the Kit:
– High sensitivity with detection limits as low as 0.005 EU/mL
– Wide linear range for accurate quantification
– Rapid results within 60 minutes
– Compatibility with various sample types
– Standardized against international reference standards
## Applications of the Chromogenic Endotoxin Quant Kit
This versatile kit finds applications in multiple fields:
### Pharmaceutical Industry
Ensuring the safety of injectable drugs and medical devices by detecting endotoxin contamination.
### Biotechnology
Monitoring endotoxin levels in recombinant protein production and cell culture systems.
### Water Quality Testing
Detecting bacterial contamination in water supplies and purification systems.
## How the Kit Works
The Chromogenic Endotoxin Quant Kit operates through a series of enzymatic reactions:
– Endotoxins activate a clotting enzyme in the presence of specific factors
– The activated enzyme cleaves a chromogenic substrate
– The resulting color change is proportional to the endotoxin concentration
– Absorbance is measured at 405-410 nm for quantification
## Advantages Over Traditional Methods
Compared to the traditional Limulus Amebocyte Lysate (LAL) test, this chromogenic method offers:
– Improved precision and reproducibility
– Reduced interference from sample components
– Quantitative results rather than just qualitative
– Easier standardization across laboratories
Keyword: Chromogenic Endotoxin Quant Kit
## Technical Specifications
Parameter | Specification
Detection Range | 0.005-50 EU/mL
Incubation Time | 60 minutes at 37°C
Sample Volume | 50-100 μL
Storage Conditions | 2-8°C
## Conclusion
The Chromogenic Endotoxin Quant Kit represents a significant advancement in bacterial toxin detection technology. Its accuracy, sensitivity, and ease of use make it an essential tool for quality control in industries where endotoxin contamination poses serious health risks. By providing reliable quantitative results, this kit helps ensure product safety and regulatory compliance across multiple applications.
