Endotoxin Quantification Kit for Chromogenic Detection

# Endotoxin Quantification Kit for Chromogenic Detection

## Introduction to Chromogenic Endotoxin Quant Kit

The Chromogenic Endotoxin Quant Kit is a powerful tool designed for accurate and sensitive detection of endotoxin levels in various samples. This kit utilizes a chromogenic method that provides reliable results with excellent reproducibility, making it an essential solution for quality control in pharmaceutical, medical device, and biotechnology industries.

## Key Features of the Kit

– High sensitivity with detection limits as low as 0.005 EU/mL
– Wide dynamic range for comprehensive analysis
– Simple and rapid procedure with results in approximately 1 hour
– Excellent correlation with LAL gel-clot method
– Suitable for various sample types including water, buffers, and biological fluids

## Principle of Chromogenic Detection

The Chromogenic Endotoxin Quant Kit works based on the enzymatic reaction of Limulus Amebocyte Lysate (LAL) with endotoxin. When endotoxin is present, it activates a cascade of enzymes in the LAL reagent, ultimately leading to the cleavage of a synthetic chromogenic substrate. This reaction produces a yellow color that can be measured spectrophotometrically at 405 nm.

## Applications of the Kit

This endotoxin quantification kit finds applications in multiple fields:

– Pharmaceutical quality control for parenteral drugs
– Medical device testing
– Water quality monitoring
– Research and development in biotechnology
– Validation of depyrogenation processes

## Advantages Over Traditional Methods

Compared to traditional gel-clot methods, the Chromogenic Endotoxin Quant Kit offers several advantages:

– Quantitative results instead of endpoint determination
– Higher throughput capability
– Better precision and accuracy
– Objective measurement via spectrophotometry
– Reduced operator-dependent variability

## Technical Specifications

Parameter | Specification
Detection Range | 0.005-1.0 EU/mL (extendable with dilution)
Incubation Time | 60 minutes at 37°C
Sample Volume | 50-100 μL
Measurement | 405 nm absorbance
Kit Components | LAL reagent, chromogenic substrate, control standard endotoxin, buffers

## Conclusion

The Chromogenic Endotoxin Quant Kit represents a significant advancement in endotoxin detection technology. Its combination of sensitivity, accuracy, and ease of use makes it an indispensable tool for industries requiring rigorous endotoxin testing. By providing quantitative results through a chromogenic method, this kit ensures compliance with regulatory standards while improving laboratory efficiency.